期刊
JOURNAL OF MAGNETIC RESONANCE
卷 242, 期 -, 页码 33-40出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2014.02.003
关键词
E.COSY; One-bond proton-carbon coupling constants; Two-bond proton-proton coupling constants; Methylene spin systems; Inverse INEPT; Residual dipolar couplings
资金
- MINECO [CTQ2012-32436]
The C-HA cross-peak corresponding to a diastereotopic CHAHB methylene spin system exhibits a characteristic 1:0:1 multiplet pattern along the indirect dimension of a omega(1)-coupled HSQC spectrum. It is shown here that the use of the initial C-13 Boltzmann polarization instead of the regular INEPT-based 1H Boltzmann polarization makes visible the central lines of this multiplet pattern. A spin-state-selective method is proposed for the efficient measurement of both (1)J(CHA) and (1)J(CHB) along the indirect dimension of a 2D spectrum as well as to the magnitude and the sign of the geminal (2)J(HAHB) coupling constant from the straightforward analysis of a single four-component E.COSY cross-peak. Additionally, the extraction of (1)J(CH) values for CH and CH3 multiplicities can be also performed from the same spectrum. The success of the method is also illustrated for the determination of residual dipolar D-1(CH) and D-2(HH) coupling constants in a small molecule weakly aligned in a PMMA swollen gel. (C) 2014 Elsevier Inc. All rights reserved.
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