期刊
JOURNAL OF MAGNETIC RESONANCE
卷 215, 期 -, 页码 115-119出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2011.12.004
关键词
Dynamic nuclear polarization; Hydration dynamics; Hyperpolarization; Overhauser effect; Polymer-lipid interaction
资金
- UCSB NSF-MRSEC [DMR05-20415]
- NSF Faculty Early CAREER [CHE-0645536]
- Packard Fellowship for Science and Engineering
- March of Dimes [6-FY07-357]
- NSF [MCB-0920316]
- University of Chicago NSF-MRSEC [DMR-0820054]
We introduce a newly developed tool, H-1 Overhauser Dynamic Nuclear Polarization (ODNP), to sensitively explore weak macromolecular interactions by site-specifically probing the modulation of the translational dynamics of hydration water at the interaction interface, in the full presence of bulk water. Here, ODNP is employed on an illustrative example of a membrane-active triblock copolymer, poloxamer 188 (P188), which is known to restore the integrity of structurally compromised cell membranes. We observe a distinct change in the translational dynamics of the hydration layer interacting with the lipid membrane surface and the bilayer-interior as P188 is added to a solution of lipid vesicles, but no measurable changes in the dynamics or structure of the lipid membranes. This study shows that hydration water is an integral constituent of a lipid membrane system, and demonstrates for the first time that the modulation of its translational diffusivity can sensitively report on weak polymer-membrane interactions, as well as mediate essential lipid membrane functions. ODNP holds much promise as a unique tool to unravel molecular interactions at interfaces even in the presence of bulk water under ambient conditions. (C) 2011 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据