期刊
JOURNAL OF MAGNETIC RESONANCE
卷 200, 期 2, 页码 291-295出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2009.07.015
关键词
Pulsed gradient spin echo; PGSE; Membrane permeability; Intracellular lifetime; Yeast
资金
- Swedish Foundation for Strategic Research (SSF)
- Swedish Research Council through the Linnaeus Center of Excellence on Organizing Molecular Matter
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A diffusion filter and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C. (C) 2009 Elsevier Inc. All rights reserved.
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