Properties of Immobilized Laccase on Mesostructured Cellular Foam Silica and its Use in Dye Decolorization

Laccase was immobilized on mesostructured cellular foam (MCF), a kind of mesoporous silica with large pore size by adsorption-cross linking method. The effects of immobilization time, temperature, pH, amount of enzyme and content of glutaraldehyde on the immobilization were optimized. The activities and stabilities towards pH and temperature of the immobilized enzyme were studied, and significantly improved enzymatic properties and good operational stability were obtained for the immobilized laccase. Dye decolorization tests showed that the immobilized enzyme could decolorize Alizarin Red and Indigo Blue solution fast and efficiently in the presence of ABTS.