Rhodamine 800 as reference substance for fluorescence quantum yield measurements in deep red emission range

The determination of fluorescence quantum yields (Phi(f)) of deep red dyes emitting at 635-900 nm is difficult due to lack of suitable standards. In this work, we propose a commercial dye, rhodamine 800 (Rho800), as reference standard which belongs to the family of xanthenes. The quantum yield of rhodamine 800 in absolute ethanol has been studied using a relative method with cresyl violet (CV) and rhodamine 101 (Rho101) as references, and an absolute fluorometric method by integrating sphere measurements. (c) 2012 Elsevier B.V. All rights reserved.