Cell labeling and cytotoxicity of aqueously synthesized CdTe/CdS/ZnS core-shell-shell quantum dots by a water bath-hydrothermal method

CdTe/CdS/ZnS core-shell-shell quantum dots (QDs) were synthesized in aqueous solution via water-bathing combined hydrothermal method using L-cysteine as a stabilizer. The present method features markedly reduced synthesis time, higher fluorescent intensity and lower cytotoxicity of the QDs. Structural and spectroscopic properties of core-shell-shell QDs are well characterized by absorption and fluorescence spectroscopy, X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. Both CdS and ZnS shells were capped on the CdTe core and the fluorescence was greatly enhanced by the ZnS coating. The ternary QDs conjugated with transferrins were successfully employed for the biolabeling and fluorescent imaging of HeLa cells. Cytotoxicity evaluation shows that CdTe/CdS/ZnS was less toxic for cells than CdTe and CdTe/CdS due to the presence of a ZnS coating on surface, which inhibited the release of cadmium ions. (C) 2011 Elsevier B.V. All rights reserved.