期刊
JOURNAL OF LUMINESCENCE
卷 132, 期 6, 页码 1522-1528出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jlumin.2012.01.021
关键词
Pharmacologically important flavonoids; Hesperitin; Hemoglobin binding; Fluorescence lifetime; Molecular modeling
类别
资金
- NIH/NCMHHD/RIMI [1P20MD002725]
- HBCU-UP
- NSF at Tougaloo College [0811638]
- CSIR, India
- Division Of Human Resource Development
- Direct For Education and Human Resources [0811638] Funding Source: National Science Foundation
- EPSCoR
- Office Of The Director [1006983, 903787] Funding Source: National Science Foundation
Hesperitin, a ubiquitous bioactive flavonoid abundant in citrus fruits is known to possess antioxidant, anti-carcinogenic, hypolipidemic, vasoprotective and other important therapeutic properties. Here we have explored the interactions of hesperitin with normal human hemoglobin (HbA), using steady state and time resolved fluorescence spectroscopy, far UV circular dicroism (CD) spectroscopy, combined with molecular modeling computations. Specific interaction of the flavonoid with HbA is confirmed from flavonoid-induced static quenching which is evident from steady state fluorescence as well as lifetime data. Both temperature dependent fluorescence measurements and molecular docking studies reveal that apart from hydrogen bonding and van der Waals interactions, electrostatic interactions also play crucial role in hesperitin-HbA interactions. Furthermore, electrostatic surface potential calculations indicate that the hesperitin binding site in HbA is intensely positive due to the presence of several lysine and histidine residues. (C) 2012 Elsevier B.V. All rights reserved.
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