期刊
JOURNAL OF LUMINESCENCE
卷 132, 期 5, 页码 1290-1298出版社
ELSEVIER
DOI: 10.1016/j.jlumin.2011.12.075
关键词
Synthesis; Derivatives; Fluorescence; Bovine serum albumin; X-ray
类别
资金
- National Natural Science Foundation of China [20962002, 20662001]
Four novel p-hydroxycinnamic acid amides, (E)-4-(3-oxo-3-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)amino)-prop-1-en-1-yl)phenyl-acetate (SPPA), (E)-3-(4-hydroxyphenyl)-N-(4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl) acrylamide (SPAA), (E)-4-(34(4-(N-(4,6-dimethylpyrimidin-2-yl)sulfamoyl)phenyl)amino)-3-oxoprop-1-en-1-yl)phenyl acetate (SPOA), and (E)-N-(4-(N-(4,6-dimethylpyrimidin-2-yl)sulfamoyl)phenyl)-3-(4-hydroxyphenyl)acrylamide (SPHA), were synthesized. The chemical structures of these compounds were confirmed using H-1-NMR. ESI-MS, and elemental analyses. SPPA and SPOA were determined using single-crystal X-ray diffraction analysis. The interactions of these four compounds with bovine serum albumin (BSA) were investigated through fluorescence and UV-vis spectral studies. The thermodynamic parameters (i.e., Delta H, Delta G, and Delta S) and the quenching constants were calculated. The binding constants and the number of binding sites, n, were investigated. The distances between BSA and its derivatives were obtained based on fluorescence resonance energy transfer, and the conformational changes in BSA were observed from synchronous fluorescence spectra. (C) 2012 Elsevier B.V. All rights reserved.
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