期刊
JOURNAL OF LUMINESCENCE
卷 131, 期 4, 页码 727-731出版社
ELSEVIER
DOI: 10.1016/j.jlumin.2010.11.026
关键词
Neodymium; Nanolabel; Luminescence; Immunoassay; Lipoprotein
类别
资金
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- Brazilian Molecular and Interfaces Nanotechnology Network CNPq
- Brazilian Institute of Nanotechnology for Integrated Markers CNPq
Neodymium based fluorescence presents several advantages in comparison to conventional rare earth or enzyme-substrate based fluorescence emitting sources (e.g.Tb, HRP). Based on this fact we have herein explored a Nd-based fluoroimmunoassay. We efficiently detected the presence of an oxidized low-density lipoprotein (oxLDL) in human plasma a well-known marker for cardiovascular diseases, which causes around 30% of deaths worldwide. Conventional fluoroimmunoassay uses time-resolved luminescence techniques, with detection in the visible range, to eliminate the fluorescence background from the biological specimens. By using an immunoassay based on functionalized Y2O3:Nd3+ nanoparticles, where the excitation and emission processes in the Nd3+ ion occur in the near-infrared (NIR) region, we have succeeded in eliminating the interferences from the biological fluorescence background, avoiding the use of time-resolved techniques. This yields higher emission intensity from the Nd3+-nanolabels and efficient detection of anti-oxidized low-density lipoproteins (anti-oxLDL) by Y2O3:Nd3+-antibody-antigen conjugation, leading to a novel biolabeling method. (C) 2010 Elsevier B.V. All rights reserved.
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