期刊
JOURNAL OF LUMINESCENCE
卷 130, 期 8, 页码 1481-1484出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jlumin.2010.03.016
关键词
Solid-state electrochemiluminescence; biosensing switch; Molecular beacon; DNA hybridization; Ferrocene; Quenching effect
类别
资金
- National Nature Science Foundation of China [20675031]
A solid-state electrochemiluminescence (ECL) biosensing switch incorporating quenching of ECL of ruthenium(II) tris-(bipyridine) (Ru(bpy)(3)(2+).) by ferrocene (Fc) has been successfully developed for DNA hybridization detection. The important issue for this biosensing system is based on the ferrocene-labeled molecular beacon (Fc-MB), i.e. using the special Fc-MB to react with the target DNA and then change its structure, resulting in an ECL intensity change. Under the optimal conditions, the difference of ECL intensity before and after the hybridization reaction (Delta I-ECL) was linearly related to the concentration of the complementary sequence in the range of 10 fM-10 pM and the detection limit was down to 1.0 fM. (C) 2010 Elsevier B.V. All rights reserved.
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