Simultaneous determination of alkaloids and flavonoids in HMC05 preparation by HPLC-DAD

High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for simultaneous determination of hesperidin, coptisine, palmatine, and berberine in HMC05, a standardized extract of eight different herbs. This method was validated in terms of specificity, linearity (r(2) > 0.9995), precision (< 5.0% RSD), and recoveries (96.6-110.5%). The LOD of these compounds were ranged from 101.6 to 171.7ng. In addition, these four compounds in HMC05 were identified or tentatively characterized using HPLC-DAD-electrospray mass spectrometry (HPLC-DAD-ESI-MS).