期刊
JOURNAL OF LIPID RESEARCH
卷 55, 期 11, 页码 2287-2295出版社
ELSEVIER
DOI: 10.1194/jlr.M050286
关键词
affinity purification; egg; endocytosis; oocyte; oogenesis; ovary; vitellogenesis; very low density lipoprotein receptor; yolk; low density lipoprotein receptor-related protein 13
资金
- North Carolina Sea Grant [R/AF-49, R/MG-0609, R/12-SSS-3]
- North Carolina Agricultural Foundation, Inc.
- Grants-in-Aid for Scientific Research [26450280] Funding Source: KAKEN
Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employing an immobilized mixture of vitellogenins Aa and Ab. RT-PCR revealed lrp13 to be predominantly expressed in striped bass ovary, and in situ hybridization detected lrp13 transcripts in the ooplasm of early secondary growth oocytes. Quantitative RT-PCR confirmed peak lrp13 expression in the ovary during early secondary growth. Quantitative mass spectrometry revealed peak Lrp13 protein levels in striped bass ovary during late-vitellogenesis, and immunohistochemistry localized Lrp13 to the oolemma and zona radiata of vitellogenic oocytes. Previously unreported orthologs of lrp13 were identified in genome sequences of fishes, chicken (Gallus gallus), mouse (Mus musculus), and dog (Canis lupus familiaris). Zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus) lrp13 loci are discrete and share genomic synteny. The Lrp13 appears to function as a vitellogenin receptor and may be an important mediator of yolk formation in fishes and other oviparous vertebrates.jlr The presence of lrp13 orthologs in mammals suggests that this lipoprotein receptor is widely distributed among vertebrates, where it may generally play a role in lipoprotein metabolism.
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