期刊
JOURNAL OF LIPID RESEARCH
卷 55, 期 11, 页码 2343-2353出版社
ELSEVIER
DOI: 10.1194/jlr.M051821
关键词
liquid chromatography; matrix-assisted laser desorption/ionization-time-of-flight; electrospray ionization-mass spectrometry; tandem mass spectrometry; galactosylceramide I-3-sulfate; lactosylceramide II3-sulfate; cortex; medulla; papillae
资金
- (Zentren fur Angewandte Forschung an Hochschulen: Applied Biomedical Mass Spectrometry, ZAFH ABIMAS) from ZO IV by the Landesstiftung Baden-Wurttemberg
- Europaischer Fonds fur regionale Entwicklung (EFRE)
Owing to its capability of discriminating subtle mass-altering structural differences such as double bonds or elongated acyl chains, MALDI-based imaging MS (IMS) has emerged as a powerful technique for analysis of lipid distribution in tissue at moderate spatial resolution of about 50 mu m. However, it is still unknown if MS1-signals and ion intensity images correlate with the corresponding apparent lipid concentrations. Analyzing renal sulfated glycosphingolipids, sulfatides, we validate for the first time IMS-signal identities using corresponding sulfatide-deficient kidneys. To evaluate the extent of signal quenching effects interfering with lipid quantification, we surgically dissected the three major renal regions (papillae, medulla, and cortex) and systematically compared MALDI IMS of renal sulfatides with quantitative analyses of corresponding lipid extracts by on-target MALDI TOF-MS and by ultra-performance LC-ESI-(triplequadrupole) tandem MS. Our results demonstrate a generally strong correlation (R-2 > 0.9) between the local relative sulfatide signal intensity in MALDI IMS and absolute sulfatide quantities determined by the other two methods. However, high concentrations of sulfatides in the papillae and medulla result in an up to 4-fold signal suppression.jlr In conclusion, our study suggests that MALDI IMS is useful for semi-quantitative dissection of relative local changes of sulfatides and possibly other lipids in tissue.
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