期刊
JOURNAL OF LIPID RESEARCH
卷 55, 期 1, 页码 155-162出版社
ELSEVIER
DOI: 10.1194/jlr.D040790
关键词
hybrid solid-phase extraction-precipitation; cardiovascular disease; hydroxycholesterol
资金
- Korea Institute of Science and Technology Institutional Program [2E24080]
- Converging Research Center Program through the Ministry of Education, Science, and Technology [2013K000427]
Alterations of cholesterol metabolism are responsible for vasospastic angina and atherosclerosis. To comprehensively evaluate cholesterol metabolism, 18 sterols, including cholesterol, 6 cholesteryl esters (CEs), 3 cholesterol precursors, and 8 hydroxycholesterols (OHCs), were simultaneously analyzed using hybrid solid-phase extraction (SPE) purification coupled to high-temperature gas chromatography-mass spectrometry (HTGC-MS). Methanol-based hybrid SPE increased the selective extraction, and HTGC resulted in a good chromatographic resolution for the separation of lipophilic compounds. The limits of quantification of cholesterol and CEs ranged from 0.2 to 10.0 mu g/ml, while OHCs and cholesterol precursors ranged from 0.01 to 0.10 mu g/ml. Linearity as the correlation coefficient was higher than 0.99 with the exception of cholesteryl laurate, myristate, oleate, and linoleate (r(2) > 0.98). The precision (%coefficient of variation) and accuracy (%bias) ranged from 1.1 to 9.8% and from 75.9 to 125.1%, respectively. The overall recoveries of CEs ranged from 26.1 to 64.0%, and the recoveries of other sterols ranged from 83.8 to 129.3%. The cholesterol signatures showed sex differences in patients with vasospastic angina and may associate with 24-reductases. This technique can be useful for making clinical diagnoses and for an increased understanding of the pathophysiology of vasospastic angina.
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