期刊
JOURNAL OF LIPID RESEARCH
卷 53, 期 8, 页码 1636-1645出版社
ELSEVIER
DOI: 10.1194/jlr.M027482
关键词
green algae; sterol evolution; cycloartenol; membranes; sterol C24-methyl transferase; sterol C25-reductase
资金
- National Science Foundation Grant [MCB-0929212]
- National Institutes of Health Grant [GM-25661]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0920212] Funding Source: National Science Foundation
Ergosterol is the predominant sterol of fungi and green algae. Although the biosynthetic pathway for sterol synthesis in fungi is well established and is known to use C24-methylation-C24 (28)-reduction (Delta(24(28))-olefin pathway) steps, little is known about the sterol pathway in green algae. Previous work has raised the possibility that these algae might use a novel pathway because the green alga Chlamydomonas reinhardtii was shown to possess a mevalonate-independent methylerythritol 4-phosphate not present in fungi. Here, we report that C. reinhardtii synthesizes the protosterol cycloartenol and converts it to ergosterol (C24 beta-methyl) and 7-dehydroporiferasterol (C24 beta-ethyl) through a highly conserved sterol C24- methylation-C25-reduction (Delta(25(27))-olefin) pathway that is distinct from the well-described acetate-mevalonate pathway to fungal lanosterol and its conversion to ergosterol by the Delta(24) ((28))-olefin pathway. We isolated and characterized 23 sterols by a combination of GC-MS and proton nuclear magnetic resonance spectroscopy analysis from a set of mutant, wild-type, and 25-thialanosterol-treated cells. The structure and stereochemistry of the final C24- alkyl sterol side chains possessed different combinations of 24 beta-methyl/ethyl groups and Delta(22(23)) E and Delta(25 (27))-double bond constructions. When incubated with [methyl-H-2(3)] methionine, cells incorporated three (into ergosterol) or five (into 7-dehydroporiferasterol) deuterium atoms into the newly biosynthesized 24 beta-alkyl sterols, consistent only with a Delta(25 (27))-olefin pathway.(jlr) Thus, our findings demonstrate that two separate isoprenoid-24-alkyl sterol pathways evolved in fungi and green algae, both of which converge to yield a common membrane insert ergosterol.-Miller, M. B., B. A. Haubrich, Q. Wang, W. J. Snell, and W. D. Nes. Evolutionarily conserved Delta(25(27))-olefin ergosterol biosynthesis pathway in the alga Chlamydomonas reinhardtii. J. Lipid Res. 2012. 53: 1636-1645.
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