期刊
JOURNAL OF LIPID RESEARCH
卷 54, 期 2, 页码 482-495出版社
ELSEVIER
DOI: 10.1194/jlr.M032771
关键词
beta-carotene; carotenoid absorption; carotenoid transport; cocoon color; lutein; positional cloning; SR-BI
资金
- Teimei Empress Memorial Foundation (Japan)
- Futaba Electronics Memorial Foundation (Japan)
- JSPS KAKENHI (Japan) [21380045, 22770138]
- Insect Technology Project of the Ministry of Agriculture, Forestry, and Fisheries (Japan)
- National Bioresource Project (Silkworm) of the Ministry of Education, Culture, Sports, Science, and Technology (Japan)
- Grants-in-Aid for Scientific Research [21380045, 22770138] Funding Source: KAKEN
Dietary carotenoids are absorbed in the intestine and delivered to various tissues by circulating lipoproteins; however, the mechanism underlying selective delivery of different carotenoid species to individual tissues remains elusive. The products of the Yellow cocoon (C) gene and the Flesh (F) gene of the silkworm Bombyx mori determine the selectivity for transport of lutein and beta-carotene, respectively, to the silk gland. We previously showed that the C gene encodes Cameo2, a CD36 family member, which is thought to function as a transmembrane lipoprotein receptor. Here, we elucidated the molecular identity of the F gene product by positional cloning, as SCRB15, a paralog of Cameo2 with 26% amino acid identity. In the F mutant, SCRB15 mRNA structure was severely disrupted, due to a 1.4 kb genomic insertion in a coding exon. Transgenic expression of SCRB15 in the middle silk gland using the binary GAL4-UAS expression system enhanced selective beta-carotene uptake by the middle silk gland, while transgenic expression of Cameo2 enhanced selective lutein uptake under the same GAL4 driver.(jlr) Our findings indicate that divergence of genes in the CD36 family determines the selectivity of carotenoid species uptake by silk gland tissue and that CD36-homologous proteins can discriminate among carotenoid species.-Sakudoh, T., S. Kuwazaki, T. Iizuka, J. Narukawa, K. Yamamoto, K. Uchino, H. Sezutsu, Y. Banno, and K. Tsuchida. CD36 homolog divergence is responsible for the selectivity of carotenoid species migration to the silk gland of the silkworm Bombyx mori. J. Lipid Res. 2013. 54: 482-495.
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