期刊
JOURNAL OF LIPID RESEARCH
卷 51, 期 6, 页码 1273-1283出版社
ELSEVIER
DOI: 10.1194/jlr.M000406
关键词
lipid fluidity; postprandial state; structural conformation; very low density lipoprotein
资金
- Treadwell Innovative Research
- Center for Health and Nutrition Research
- National Institutes of Health [HL-HL71488, HL-55667, RO1 AG 028793, R01 AG029246]
- Hungarian National Research Fund [OTKA T048334]
- National Center for Research Resources, National Institutes of Health [C06 RR-12088-01]
- NATIONAL CENTER FOR RESEARCH RESOURCES [C06RR012088] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL055667, R01HL071488] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG028793, R01AG029246, R01AG039094] Funding Source: NIH RePORTER
Our previous work indicated that apolipoprotein (apo) E4 assumes a more expanded conformation in the postprandial period. The postprandial state is characterized by increased VLDL lipolysis. In this article, we tested the hypothesis that VLDL lipolysis products increase VLDL particle fluidity, which mediates expansion of apoE4 on the VLDL particle. Plasma from healthy subjects was collected before and after a moderately high-fat meal and incubated with nitroxyl-spin labeled apoE. ApoE conformation was examined by electron paramagnetic resonance spectroscopy using targeted spin probes on cysteines introduced in the N-terminal (S76C) and C-terminal (A241C) domains. Further, we synthesized a novel nitroxyl spin-labeled cholesterol analog, which gave insight into lipoprotein particle fluidity. Our data revealed that the order of lipoprotein fluidity was HDL similar to LDL
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