期刊
JOURNAL OF LIPID RESEARCH
卷 50, 期 3, 页码 501-513出版社
ELSEVIER
DOI: 10.1194/jlr.M800426-JLR200
关键词
lipids; meibomian glands; HPLC; mass spectrometry
资金
- Research to Prevent Blindness (New York, NY)
- National Institutes of Health [EY-016664]
Human meibomian gland secretions (also known as meibum) were analyzed for the presence of cholesteryl esters (Chl-E) using HPLC in combination with atmospheric pressure chemical ionization mass spectrometry. A special procedure based on detection of the in-source generated ion m/z 369 was developed to monitor all Chl-E simultaneously. The structures of the detected compounds were studied using in-source and postsource fragmentation of the precursor (M+H)(+) ions. In concordance with previous studies, Chl-E were found in all of the tested samples and comprised similar to 31% of the entire lipid pool (w/w, dry weight). There were at least 20 different saturated and unsaturated Chl-E species observed, whose fatty acid residues ranged from C18 to C34. Monounsaturated fatty acids were the most visible components of the Chl-E pool. The eleven most prominent compounds were: C20:0-, C22:1-, C22:0-, C24:1-, C24:0, C25:0-, C26:1-, C26:0-, C28:1-, C28:0-, and C30:1-Chl-E. Other Chl-containing compounds were detected but not identified at the time. Therefore, Chl-E are a depot for very long chain saturated and monounsaturated fatty acids in human meibum.-Butovich, I. A. Cholesteryl esters as a depot for very long chain fatty acids in human meibum. J. Lipid Res. 2009. 50: 501-513.
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