期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 94, 期 4, 页码 791-802出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0113035
关键词
granulocytes; hydrocortisone; LPS; shedding
资金
- Swiss National Science Foundation [310030_135195, 310030_134691]
- Rheumasearch Foundation
- Institute of Arthritis Research
- Swiss National Science Foundation (SNF) [310030_135195, 310030_134691] Funding Source: Swiss National Science Foundation (SNF)
The proinflammatory activities of IL-1 are tightly controlled at different levels. IL-1R2 acts as a decoy receptor and has been shown to regulate the biological effects of IL-1 in vitro and in vivo. However, little is known about its natural expression in the mouse in physiologic and pathologic conditions. In this study, we examined IL-1R2 mRNA and protein expression in isolated cells and tissues in response to different stimulatory conditions. Data obtained using ex vivo CD11b(+)Ly6G(+) peripheral blood cells and in vitro-differentiated CD11b(+)Ly6G(+) BMG indicated that neutrophils are the major source of constitutively expressed IL-1R2 in the mouse. The expression of IL-1R2 on BMG and ex vivo Ly6G(+) peripheral blood cells was highly up-regulated by HC. IL-1R2 pull-down experiments showed that mouse rIL-1 beta binds to BMG IL-1R2, whereas binding of IL-1Ra could not be detected. Furthermore, LPS treatment induced shedding of IL-1R2 from the neutrophil membrane in vitro and in vivo, executed mainly by ADAM17. Finally, in in vivo models of inflammation, including thioglycolate-induced acute peritonitis and acute lung injury, infiltrating Ly6G(+) neutrophils, expressed IL-1R2. Our data show that in the mouse, neutrophils mainly express the decoy receptor IL-1R2 under naive and inflammatory conditions. These data suggest that neutrophils may contribute to the resolution of acute inflammation.
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