期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 93, 期 1, 页码 135-143出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0112025
关键词
5-lipoxygenase; inflammation; transcellular synthesis
资金
- National Eye Institute [K08EY16833, R01EY021535]
- Medical Research Service of the U.S. Department of Veterans Affairs
- U.S. National Institutes of Health [R01EY00300, HL34303]
- NATIONAL EYE INSTITUTE [R01EY000300, R01EY021535, P30EY011373, K08EY016833] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL034303] Funding Source: NIH RePORTER
Understanding the early pathogenesis of DR may uncover new therapeutic targets to prevent or slow the progression of this sight-threatening disorder. We investigated the role of leukocyte-mediated generation of LTs in regulation of retinal capillary degeneration and inflammation in the diabetic mouse. We generated (1) chimeric mice that lacked the ability to generate LTs by transplanting 5LO-/- bone marrow cells into ND. WT mice and into SD. WT mice and (2) control chimeric mice by transplanting WT bone marrow cells into 5LO-/- mice or into WT mice. Retinas from diabetic chimeric mice with WT marrow demonstrated capillary degeneration to the same extent as retinas from diabetic, nonchimeric WT mice. In contrast, retinas from diabetic chimeric mice with 5LO-/- marrow developed significantly less capillary degeneration and pericyte loss (P<0.05). In the retinas from chimeric mice with WT marrow, diabetes induced a rise in leukocyte adherence to the microvasculature, expression of the NF-kappa B p65 subunit, and ICAM1, superoxide generation, and retinal microvascular permeability, yet these characteristic responses were blunted by >50% in diabetic chimeras containing 5LO-/- leukocytes (P<0.05). Our data suggest the critical involvement of leukocytes and LTs in the regulation of inflammation and capillary degeneration in DR. J. Leukoc. Biol. 93: 135-143; 2013.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据