期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 90, 期 3, 页码 459-470出版社
WILEY
DOI: 10.1189/jlb.0311145
关键词
eicosanoid; TNF; IL-6; resolution of inflammation
资金
- Dana Human Immunology Award
- Defense Advanced Projects Agency (DARPA)
- Greene Fund
- NIH [R21 HL089659-01A1, RO1 AI067353]
- Thrasher Foundation
- American Recovery and Reinvestmant Act NIH Administrative Supplement [3R01AI067353-05S1]
- NIH (Bethesda, MD, USA) [R01GM038765, P01GM095467]
- Dartmouth Center for Medical
Detection and clearance of bacterial infection require balanced effector and resolution signals to avoid chronic inflammation. Detection of GNB LPS by TLR4 on m(phi) induces inflammatory responses, contributing to chronic inflammation and tissue injury. LXs and Rvs are endogenous lipid mediators that enhance resolution of inflammation, and their actions on primary human m(phi) responses toward GNB are largely uncharacterized. Here, we report that LXA(4), LXB4, and RvD1, tested at 0.1-1 mu M, inhibited LPS-induced TNF production from primary human m(phi), with ATL and 17(R)-RvD1, demonstrating potent inhibition at 0.1 mu M. In addition, 17(R)RvD1 inhibited LPS-induced primary human m(phi) production of IL-7, IL-12p70, GM-CSF, IL-8, CCL2, and MIP-1 alpha without reducing that of IL-6 or IL-10. Remarkably, when stimulated with live Escherichia coli, m(phi) treated with 17(R)-RvD1 demonstrated increased TNF production and enhanced internalization and killing of the bacteria. 17(R)-RvD1-enhanced TNF, internalization, and killing were not evident for an lpxM mutant of E. coli expressing hypoacylated LPS with reduced inflammatory activity. Furthermore, 17(R)-RvD1-enhanced, E. coli-induced TNF production was evident in WT but not TLR4-deficient murine m(phi). Thus, Rvs differentially modulate primary human m(phi) responses to E. coli in an LPS- and TLR4-dependent manner, such that this Rv could promote resolution of GNB/LPS-driven inflammation by reducing m(phi) proinflammatory responses to isolated LPS and increasing m(phi) responses important for clearance of infection. J. Leukoc. Biol. 90: 459-470; 2011.
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