期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 90, 期 1, 页码 167-176出版社
WILEY
DOI: 10.1189/jlb.1110624
关键词
M2; IL-10; phagocytosis; CRTH2
资金
- National Institutes of Health [RO1 HL71711]
- DOD DAMD [17-03-1-0004]
- Bankhead-Coley Cancer Research Program [06BS-04-9615]
- Charles E. Schmidt Biomedical Foundation
Murine M phi that phagocytose CMP develop into M1; this response depends on the size and the chemical composition of the particles. In contrast, recent studies concluded that chitin particles induce M2 and eosinophil migration, promoting acquired Th2 immune responses against chitin-containing microbes or allergens. This study examined whether these apparently inconsistent responses to chitin could be induced by variation in the size and chemical composition of the chitin particles. We compared the responses of M phi with CMP, LCB, and Sephadex G-100 beads (>40 mu m). Beads were given i.p. to WT mice and to mice deficient in a CRTH2, a receptor for the eosinophil chemoattractant PGD(2). In contrast to the M1 activation induced by CMP, i.p. administration of LCB or Sephadex beads induced within 24 h a CRTH2-dependent peritoneal eosinophilia, as well as CRTH2-independent activation of peritoneal M phi that expressed Arg I, an M2 phenotype. LCB-induced M phi exhibited elevated Arg I and a surface MR, reduced surface TLR2 levels, and no change in the levels of CHI3L1 or IL-10 production. Our results indicate that the effects of chitin in vivo are highly dependent on particle size and that large, nonphagocytosable beads, independent of their chemical composition, induce innate eosinophilia and activate M phi expressing several M2, but not M1, phenotypes. J. Leukoc. Biol. 90: 167-176; 2011.
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