期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 90, 期 6, 页码 1043-1054出版社
WILEY
DOI: 10.1189/jlb.0311168
关键词
colon; inflammation; CXCR3; chemokine
资金
- NIH/NCCAM [P01 AT004986]
- NIH [HHSN2662004000009/N01-AI40009]
Human IBD, including UC and Crohn's disease, is characterized by a chronic, relapsing, and remitting condition that exhibits various features of immunological inflammation and affects at least one/1000 people in Western countries. Polyphenol extracts from a variety of plants have been shown to have immunomodulatory and anti-inflammatory effects. In this study, treatment with APP was investigated to ameliorate chemically induced colitis. Oral but not peritoneal administration of APP during colitis induction significantly protected C57BL/6 mice against disease, as evidenced by the lack of weight loss, colonic inflammation, and shortening of the colon. APP administration dampened the mRNA expression of IL-1 beta, TNF-alpha, IL-6, IL-17, IL-22, CXCL9, CXCL10, CXCL11, and IFN-gamma in the colons of mice with colitis. APP-mediated protection requires T cells, as protection was abated in Rag-1(-/-) or TCR alpha(-/-) mice but not in IL-10(-/-), IRF-1(-/-), mu MT, or TCR delta(-/-) mice. Administration of APP during colitis to TCR alpha(-/-) mice actually enhanced proinflammatory cytokine expression, further demonstrating a requirement for TCR alpha beta cells in APP-mediated protection. APP treatment also inhibited CXCR3 expression by TCR alpha beta cells, but not B or NK cells, in the colons of mice with colitis; however, depletion of CD4(+) or CD8(+) T cells alone did not abolish APP-mediated protection. Collectively, these results show that oral administration of APP protects against experimental colitis and diminishes proinflammatory cytokine expression via T cells. J. Leukoc. Biol. 90: 1043-1054; 2011.
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