期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 88, 期 3, 页码 597-603出版社
WILEY
DOI: 10.1189/jlb.0310184
关键词
macrophage; monocyte; flow cytometry; spleen
资金
- CNRS
- Institut national de la sante et de la recherche medicale
- European Community
- Australian National Health and Medical Research Council
- MPCM
Cellular AF is usually considered a hindrance to flow cytometric analysis. Here, we incorporate AF into analysis of complex mixtures of leukocytes. Using a mouse model, we examined cellular AF at multiple excitation and emission wavelengths, and populations with discrete patterns were gated and examined for surface marker expression. In the spleen, all major myeloid populations were identified. In particular, the approach allowed simultaneous characterization of RPM and resident monocytes. When monocytes and RPM were compared, RPM exhibited a phenotype that was consistent with involvement in physiological processes, including expression of genes involved in lipid and iron metabolism. The presence of large amounts of stored ferric iron within RPM enabled purification of these cells using a magnetic-based approach. When adapted for use on leukocytes isolated from a range of other organs, incorporation of AF into analysis allowed identification and isolation of biologically important myeloid populations, including subsets that were not readily identifiable by conventional cytometric analysis. J. Leukoc. Biol. 88: 597-603; 2010.
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