期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 89, 期 1, 页码 57-62出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0610381
关键词
CRAC; SOCE; STIM2; Orai2
资金
- National Institutes of Health
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R03DE019524] Funding Source: NIH RePORTER
SOCE via CRAC channels is a critical signaling event in immune cells. Recent studies have identified key proteins underlying this process; STIM is an ER Ca2+ sensor that interacts with Orai, an intrinsic, pore-forming protein of the CRAC channel. In heterologous expression systems, STIM1 regulates SOCE by interacting with Orai1, -2, and -3. In native tissues, however, the precise roles of STIM and Orai proteins are not well defined. Here, we have investigated the molecular components of SOCE signaling in mouse DCs. We show that DCs predominantly express STIM2 and only very low levels of STIM1 compared with T lymphocytes. Upon store depletion with Tg, STIM2 aggregates and interacts selectively with Orai2. In contrast, Tg fails to aggregate STIM1 or enhance STIM1-mediated interactions with Orai proteins. Consistent with this biochemical characterization, stimulation of DCs with the adhesion molecule ICAM-1 selectively recruits STIM2 and Orai2 to the IS. Together, these data demonstrate a novel, STIM2-dependent SOCE signaling pathway in DCs. J. Leukoc. Biol. 89: 57-62; 2011.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据