期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 87, 期 5, 页码 753-764出版社
WILEY
DOI: 10.1189/jlb.0909624
关键词
chicken; zebra finch; macrophage; co-evolution
资金
- BBSRC
- [BB/D010705/1]
- Biotechnology and Biological Sciences Research Council [BB/F024347/1, BB/D013704/1, BBS/B/13500, BBS/E/R/00000690, G18647, BB/D010705/1, BB/D013704/2, BB/D523578/1] Funding Source: researchfish
- Medical Research Council [G9900991B] Funding Source: researchfish
- BBSRC [BB/F024347/1, BB/D013704/2, BB/D010705/1, BB/D013704/1] Funding Source: UKRI
Macrophages are involved in many aspects of development, host defense, pathology, and homeostasis. Their normal differentiation, proliferation, and survival are controlled by CSF-1 via the activation of the CSF1R. A recently discovered cytokine, IL-34, was shown to bind the same receptor in humans. Chicken is a widely used model organism in developmental biology, but the factors that control avian myelopoiesis have not been identified previously. The CSF-1, IL-34, and CSF1R genes in chicken and zebra finch were identified from respective genomic/cDNA sequence resources. Comparative analysis of the avian CSF1R loci revealed likely orthologs of mammalian macrophage-specific promoters and enhancers, and the CSF1R gene is expressed in the developing chick embryo in a pattern consistent with macrophage-specific expression. Chicken CSF-1 and IL-34 were expressed in HEK293 cells and shown to elicit macrophage growth from chicken BM cells in culture. Comparative sequence and co-evolution analysis across all vertebrates suggests that the two ligands interact with distinct regions of the CSF1R. These studies demonstrate that there are two separate ligands for a functional CSF1R across all vertebrates. J. Leukoc. Biol. 87: 753-764; 2010.
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