Anti-Mullerian Hormone Levels in the Follicular Fluid of the Preovulatory Follicle: A Predictor for Oocyte Fertilization and Quality of Embryo

This prospective study investigated the relationship between anti-Mullerian an hormone (AMH) level in the follicular fluid (FF) and the quality of the oocyte and embryo. A total of 65 FF samples from 54 women were included in this study. FF was collected from the largest preovulatory follicle sized >= 20 mm of mean diameter from each ovary. Samples were divided into 3 groups according to the FF AMH levels: below the 33th percentile (low group, FF AMH < 2.1 ng/mL, n = 21), between the 33th and the 67th percentile (intermediate group, FF AMH = 2.1-3.6 ng/mL, n = 22), and above the 67th percentile (high group, FF AMH > 3.6 ng/mL, n = 22). The quality of the ensuing oocytes and embryos was evaluated by fertilization rate and embryo score. FF AMH levels correlated positively with the matched embryo score on day 3 after fertilization (r = 0.331, P = 0.015). The normal fertilization rate was significantly lower in the low group than in the intermediate group (61.9% vs. 95.5% vs. 77.3%, respectively, P = 0.028). Our results suggest that the FF AMH level could be a predictor of the ensuing oocyte and embryo quality.