期刊
JOURNAL OF INVESTIGATIVE SURGERY
卷 21, 期 3, 页码 101-108出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/08941930802043565
关键词
tight junction; ZO-2; blood-brain barrier permeability; brain edema; acute liver failure
类别
资金
- NIDDK NIH HHS [R21 DK064361, DK064361, R21 DK064361-01, R21 DK064361-02] Funding Source: Medline
- NINDS NIH HHS [R01 NS051646, R01 NS051646-02, R01 NS051646-01A2, R01NS051646] Funding Source: Medline
Brain edema secondary to increased blood-brain barrier (BBB) permeability is a lethal complication in fulminant hepatic failure (FHF). Intact tight junctions (TJ) between brain capillary endothelial cells are critical for normal BBB function. However, the role of TJ in FHF has not been explored. We hypothesized that alterations in the composition of TJ proteins would result in increased BBB permeability in FHF. In this study, FHF was induced in C57BL/6J mice by using azoxymethane. BBB permeability was assessed with sodium fluorescein. Expression of TJ proteins was determined by Western blot, and their cellular distribution was examined using immunofluorescent microscopy. Comatose FHF mice had significant cerebral sodium fluorescein extravasation compared with control and precoma FHF mice, indicating increased BBB permeability. Western blot analysis showed a significant decrease in zonula occludens (ZO)-2 expression starting in the precoma stage. Immunofluorescent microscopy showed a significantly altered distribution Pattern of ZO-2 in isolated microvessels from precoma FHF mice. These changes were more prominent in comatose FHF animals. Significant alterations in ZO-2 expression and distribution in the tight junctions preceded the increased BBB permeability in FHF mice. These results suggest that ZO-2 may play an important role in the pathogenesis of brain edema in FHF.
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