期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 139, 期 2, 页码 342-351出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jid.2018.07.033
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资金
- Austrian Science Fund [FWF: V 525-B28]
- Federation of European Biochemical Societies (FEBS, Follow-Up Research Fund)
- DOC Fellowship of the Austrian Academy of Sciences (OeAW)
Human skin dermis is composed of the superficial papillary dermis and the reticular dermis in the lower layers, which can easily be distinguished histologically. In vitro analyses of fibroblasts from explant cultures from superficial and lower dermal layers suggest that human skin comprises at least two fibroblast lineages with distinct morphology, expression profiles, and functions. However, while for mouse skin cell surface markers have been identified, allowing the isolation of pure populations of one lineage or the other via FACS, this has not been achieved for human skin fibroblasts. We have now discovered two cell surface markers that discriminate between papillary and reticular fibroblasts. While FAP thorn CD90 e cells display increased proliferative potential, express PDPN and NTN1, and cannot be differentiated into adipocytes, FAP e CD90 thorn fibroblasts express high levels of ACTA2, MGP, PPARg, and CD36 and readily undergo adipogenic differentiation, a hallmark of reticular fibroblasts. Flow cytometric analysis of fibroblasts isolated from superficial and lower layers of human dermis showed that FAP thorn CD90 e cells are enriched in the papillary dermis. Altogether, functional analysis and expression profiling confirms that FAP thorn CD90 e cells represent papillary fibroblasts, whereas FAP e CD90 thorn fibroblasts derive from the reticular lineage. Although papillary and reticular fibroblasts are enriched in the upper or lower dermis, respectively, they are not spatially restricted, and the microenvironment seems to affect their function.
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