期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 134, 期 7, 页码 1862-1872出版社
ELSEVIER SCIENCE INC
DOI: 10.1038/jid.2014.90
关键词
-
类别
资金
- Canadian Institutes of Health Research [286720, 210820]
- Collaborative Health Research Programme (CIHR/NSERC) [1004005, 413783]
- Canada Foundation for Innovation and Ontario Research Fund [26653]
- Heart and Stroke Foundation Ontario [NA7086]
- Generalitat de Catalunya
- Novartis Stiftung fur Biologisch-Medizinische Forschung
- Service Academique, EPFL
- Competence Centre for Materials Science and Technology (CCMX) of the ETH-Board, Switzerland
Changes in the mechanical properties of dermis occur during skin aging or tissue remodeling and affect the activity of resident fibroblasts. With the aim to establish elastic culture substrates that reproduce the variable softness of dermis, we determined Young's elastic modulus E of human dermis at the cell perception level using atomic force microscopy. The E of dermis ranged from 0.1 to 10 kPa, varied depending on body area and dermal layer, and tended to increase with age in 26-55-year-old donors. The activation state of human dermal fibroblasts cultured on skin-soft E (5 kPa) silicone culture substrates was compared with stiff plastic culture (GPa), collagen gel cultures (0.1-9 kPa), and fresh human dermal tissue. Fibroblasts cultured on skin-soft silicones displayed low mRNA levels of fibrosis-associated genes and increased expression of the matrix metalloproteinases (MMPs) MMP-1 and MMP-3 as compared with collagen gel and plastic cultures. The activation profile exhibited by fibroblasts on skin-soft silicone culture substrates was most comparable with that of human dermis than any other tested culture condition. Hence, providing biomimetic mechanical conditions generates fibroblasts that are more suitable to investigate physiologically relevant cell processes than fibroblasts spontaneously activated by stiff conventional culture surfaces.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据