期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 132, 期 2, 页码 401-409出版社
ELSEVIER SCIENCE INC
DOI: 10.1038/jid.2011.317
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资金
- National Institutes of Health, National Cancer Institute, Center for Cancer Research
Primary human squamous cell carcinomas (SCCas) are heterogeneous invasive tumors with proliferating outer layers and inner differentiating cell masses. To determine if tumor-initiating cells (TICs) are present in SCCas, we utilized newly developed reliable in vitro and in vivo xenograft assays that propagate human SCCas, and demonstrated that a small subset of SCCa cells (similar to 1%) expressing Prominin-1 (CD133) in the outer layers of SCCas were highly enriched for TICs (similar to 1/400) compared with unsorted SCCa cells (TICs similar to 1/10(6)). Xenografts of CD133+ SCCas recreated the original SCCa tumor histology and organizational hierarchy, whereas CD133- cells did not, and only CD133+ cells demonstrated the capacity for self-renewal in serial transplantation studies. We present a model of human SCCas in which tumor projections expand with outer leading edges that contain CD133+ TICs. Successful cancer treatment will likely require that the TICs identified in cancers be targeted therapeutically. The demonstration that TICs are present in SCCas and are enriched in a CD133- expressing subpopulation has not been, to our knowledge, previously reported.
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