期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 157, 期 -, 页码 25-31出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2018.07.012
关键词
Tetrahymena glochidiophila; Lampsilis spp.; Parasitism; Unionidae; Ciliophora
类别
资金
- Government of Canada's Natural Science and Engineering Research Council's Discovery program
- Government of Canada's Natural Science and Engineering Research Council's Visiting Post-doctoral Fellowship program
- Environment and Climate Change Canada
This study investigated the effect of a previously uncharacterized species of ciliated protozoan, Tetrahymena glochidiophila, on the viability of glochidia from three species of freshwater mussel (Lampsilis siliquoidea, Lampsilis fasciola, and Lampsilis cardium). Over the course of 72 h, the viability of glochidia exposed to T. glochidiophila declined by > 60% while the decline in the viability of uninfected glochidia was < 10%. The density of T. glochidiophila increased > 1000-fold during the experiment in treatments with infected glochidia. Lampsilis cardium glochidia were also either exposed to gill rinsate or gill contents from infected gravid female L. sillquoidea for the purpose of elucidating the location of the greatest density of ciliates within infected mussels. Glochidia exposed to gill contents declined significantly (p < 0.05) more than glochidia exposed to gill rinsate. Finally, a clone of ciliates was isolated from infected glochidia and cultured on bacterized medium. The clonal culture was then used to expose uninfected glochidia for the purpose of further confirming a parasitic relationship between glochidia and T. glochidiophila. The viability of glochidia exposed to T. glochidiophila from the clonal culture declined significantly relative to uninfected glochidia but not to the extent of glochidia exposed to ciliates from the gills of infected L. siliquoidea.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据