期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 115, 期 -, 页码 99-101出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2013.11.003
关键词
Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae); Bacillus thuringiensis; Cry9Aa5
类别
资金
- FP7 BioSUPPORT project
- Bulgarian Science Fund
Cry9Aa, produced by Bacillus thuringiensis is reported to be not active against Spodoptera exigua (beet armyworm). In this study we have cloned a new cry9Aa5 gene encoding a protoxin with increased activity against S. exigua as compared to Cry9Aa1. When aligned to Cry9Aa1, four amino acid substitutions in domain I and one substitution in the C-terminal protein extension of Cry9Aa5 were identified. Toxicity of Cry9Aa5, produced in recombinant Escherichia coli was assessed and compared to the activity of Cry9Aa1, produced under the same conditions. (C) 2013 Elsevier Inc. All rights reserved.
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