期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 115, 期 -, 页码 76-79出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2013.10.011
关键词
Gene expression; Bee virus; RT-qPCR; Dicistroviruses; qBase(PLUS)
类别
资金
- Special Research Fund of Ghent University (BOF-UGent)
- Fund for Scientific Research-Flanders (FWO-Vlaanderen, Belgium)
- China Scholarship Council (CSC)
- BOF-UGent
To date, there are no validated internal reference genes for the normalization of RT-qPCR data from virus infection experiments with pollinating insects. In this study we evaluated the stability of five candidate internal reference genes: elongation factor-1 -alpha (ELF1 alpha), peptidylprolyl isomerase A (PPIA), 60S ribosomal protein L23 (RPL23), TATA-binding protein (TBP) and polyubiquitin (UBI), in relation to Israeli acute paralysis virus (IAPV) infection of Bombus terrestris. We investigated the stability of these genes: in whole bodies and individual body parts, as well as in whole bodies collected at different time intervals after infection with IAPV. Our data identified PPIA as the single, most-optimal internal reference gene and the combination of PPAI-RPL23-UBI as a fully-sufficient multiple internal reference genes set for IAPV infection experiments in B. terrestris. (C) 2013 Elsevier Inc. All rights reserved.
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