期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 113, 期 1, 页码 35-41出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2013.01.001
关键词
Nosema; Honey bee; Tissue tropism; Glands; Transmission; Development
类别
资金
- Agriculture and Food Research Initiative, from the USDA National Institute of Food and Agriculture [20098511805718, RC293-365/4692038]
- USDA-CSREES Project [ILLU-875-302-0205249 S-1024]
- Prairie Research Institute, Illinois Natural History Survey at the University of Illinois at Urbana-Champaign
The two etiological agents of nosema disease in honey bees, Nosema apis and Nosema ceranae (Microsporidia: Nosematidae), reproduce in the midgut tissues of the host. N. apis is tissue specific but the development and tissue tropism of N. ceranae is not well understood. Our investigations compared development of the two phylogenetically related pathogens in all major host tissues. Using microscopy, PCR and qPCR quantification to evaluate tissue tropism of infected bees in communal cages and of individually restrained infected bees, we found no detectable spores in cephalic or other body tissues except midgut tissues. Nosema DNA was detected in Malpighian tubules but the tubules could not be separated from the alimentary tract without release of spores from the midgut. Nosema DNA was not detected in hemolymph sampled from the head capsule or the abdomen of infected bees. We confirmed that N. ceranae only develops in midgut tissues. Spores of both species released from host midgut cells accumulated in the hindgut lumen, and we noted differences in numbers and ratios of spore types and in growth curves between the two pathogens. N. apis reached a consistent level of spore production after 12 days post inoculation (dpi); N. ceranae spore production increased linearly from 12 to 20 dpi and the number of. mature N. ceranae spores was consistently higher. (c) 2013 Elsevier Inc. All rights reserved.
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