期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 114, 期 1, 页码 7-10出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2013.05.002
关键词
Dendronereis spp.; WSSV; Replication; Immunohistochemisuy; RT-PCR
类别
资金
- Wageningen University
This study investigated whether WSSV replicates in naturally infected Dendronereis spp., a common polychaete (Nereididae) species in shrimp ponds in Indonesia. To detect WSSV replication, (i) immunohistochemistry (IHC) using a monoclonal antibody against WSSV VP28 protein and (ii) nested RT-PCR using specific primers set for the vp28 gene to detect WSSV-specific mRNA were applied. WSSV immunoreactive-nuclei were detected in the gut epithelium of the polychaete and WSSV mRNA was detected with nested RT-PCR. This, together with the IHC results, confirmed that WSSV could replicate in Dendronereis spp. This is the first report showing that WSSV replicated. in a naturally infected non-crustacean host. (C) 2013 Elsevier Inc. All rights reserved.
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