期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 113, 期 1, 页码 52-55出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2013.01.005
关键词
Nosemosis; Multiplex PCR; Diagnostic; Nosema apis; Nosema ceranae
类别
资金
- Anses
Nosema apis and Nosema ceranae are the causative agents of nosemosis, a contagious honeybee disease that weakens bee colonies. The species are discriminated through several PCR-based methods including a multiplex PCR recommended by the World Organization for Animal Health (OIE). In this study, the OIE protocol was compared to two other PCR protocols using different PCR kits with the same primer pairs as described in OIE. The results showed that the three PCR protocols have similar sensitivity but only the kit dedicated to multiplex PCR could detect small quantities of one Nosema species when greater quantities of the other were also present. However, singleplex PCR methods are currently the most sensitive methods for discerning each species. These results have important implications for epidemiology and the understanding of the disease. (c) 2013 Elsevier Inc. All rights reserved.
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