期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 109, 期 1, 页码 127-133出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2011.10.009
关键词
Spodoptera exigua; Iflavirus; SeMNPV; RNA virus; Covert infection; Picorna-like virus
类别
资金
- Spanish Ministry of Science and Innovation [AGL2010-16809, AGL2011-30352-C02-02]
- European FEDER
Analysis of the transcriptome of Spodoptera exigua larvae revealed the presence of several ESTs with homology to virus of the order Picornavirales and with the highest similarity to Infectious flacherie virus (Iflaviridae) that infects Bombyx mori larvae. Iflaviridae is a recently defined family of insect-infecting viruses that consist of positive single strand RNA genomes translated into a single polyprotein of around 3000 amino acids long. Using the sequence information derived from the obtained ESTs. we have completed the genomic sequence of this virus. The novel S. exigua iflavirus (SeIV-1) has a genome of 10.3 kb and codes for a 3222 aa polyprotein. Expression analysis has revealed the presence of the virus in all tissues tested and insect stages, being more abundant in the midgut of the larvae. High infectivity of this virus against S. exigua has been demonstrated after observing the presence of this virus in different colonies that were reared in the same chamber with the virus-infected colony, despite no evidence of pathological effects. Further study of viral covert infections of SeIV-1 could lead to a better understanding of its pathological effect as well as any possible interaction with other microbial pathogens used for the control of this pest. (C) 2011 Elsevier Inc. All rights reserved.
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