期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 107, 期 1, 页码 11-15出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2011.01.003
关键词
Bacillus thuringiensis; Bacillus cereus; Lysinibacillus sphaericus; Culicidae larvae and bacterial biodiversity
类别
资金
- Fundacao de Amparo A Pesquisa do Estado de Sao Paulo (FAPESP) [2008/05700-2]
The aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in Sao Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba. Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatment and incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluation of biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresis of crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L-2 fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons. (C) 2011 Elsevier Inc. All rights reserved.
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