期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 98, 期 3, 页码 267-270出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2008.01.010
关键词
biological control; endophytic colonization; plant disease; Pythium myriotylum; Rhizoctonia solani; Xanthomonas axonopodis pv. malvacearum
类别
Seed application of Beauveria bassiana 11-98 resulted in endophytic colonization of tomato and cotton seedlings and protection against plant pathogenic Rhizoctonia solani and Pythium myriotylum. Both pathogens cause damping off of seedlings and root rot of older plants. The degree of disease control achieved depended upon the population density of B. bassiana conidia on seed. Using standard plating techniques onto selective medium, endophytic 11-98 was recovered from surface-sterilized roots, stems, and leaves of tomato, cotton, and snap bean seedlings grown from seed treated with B. bassiana 11-98. As the rate of conidia applied to seed increased, the proportion of plant tissues from which B. bassiana 11-98 was recovered increased. For rapid detection of B. bassiana 11-98 in cotton tissues, we developed new ITS primers that produce a PCR product for B. bassiana 11-98, but not for cotton. In cotton samples containing DNA from B. bussiana 11-98, the fungus was detected at DNA ratios of 1: 1000: B. bassiana 11-98 was detected also in seedlings grown from seed treated with B. bassiana 11-98, Using SEM, hyphae of B. bassiana 11-98 were observed penetrating epithelial cells of cotton and ramifying through palisade parenchyma and mesophyll leaf tissues. B. bassiana 11-98 induced systemic resistance in cotton against Xanthomonas axonopodis pv. malvacearum (bacterial blight). In parasitism assays, hyphae of B. bassiana 11-98 were observed coiling around hyphae of Pythium myriotylum. (C) 2008 Elsevier Inc. All rights reserved.
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