期刊
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
卷 41, 期 2, 页码 284-292出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0300060513476436
关键词
Cell adhesion; gene expression; invasiveness; metastasis; RNA interference; semaphorin; stomach neoplasm
资金
- Fundamental Research Fund for the Central Universities of Central South University, Changsha City, China
Objective: To investigate the role of semaphorin 6B in gastric cancer invasion and metastasis. Methods: Immunohistochemistry for semaphorin 6B was performed on gastric cancer tumour tissue samples in this retrospective study. Levels of semaphorin 6B protein and mRNA were determined in gastric cancer cell lines by Western blotting and quantitative reverse transcription-polymerase chain reaction, respectively. The human gastric cancer cell line SGC-7901 was transfected with small interfering RNA targeting semaphorin 6B; effects on cell adhesion, migration and invasion were determined by cell adhesion assay, transwell chamber migration assay and wound healing assay, respectively. Results: Tumour tissue samples from 220 patients were analysed. In vivo, semaphorin 6B immunopositivity correlated with tumour differentiation, lymph node metastasis and distant metastasis but not patient age, sex or tumour stage. Semaphorin 6B gene silencing significantly suppressed adhesion, migration and invasion of gastric cancer cells in vitro. Conclusions: Semaphorin 6B is related to tumour differentiation and metastasis in vivo, and tumour cell migration, adhesion and invasion in vitro. Semaphorin 6B may represent a reliable biomarker for diagnosis, evaluation and gene-targeted therapy of gastric cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据