期刊
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
卷 37, 期 3, 页码 621-630出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/147323000903700304
关键词
SIDE POPULATION; CANCER STEM CELLS; BLADDER TRANSITIONAL; CELL CANCER; T24
资金
- Chinese National Scientific Research Special Purpose [200802015]
- Chinese National Scientific [30500507, 30672092]
- 5010 Project of Sun Yat-sen University [2007018]
- Clinical Key Project of Public Health Ministry
- Yat-sen Scholarship for Young Scientists
Cancer stem cells can be isolated from human tumours using specific cell surface markers. Bladder cancer cells, however, lack specific cell surface markers, making this approach impracticable. In this study an alternative method was used, involving isolation of side population cells to explore the stem cell characteristics of bladder cancer. Side population cells were isolated from the bladder transitional cell cancer cell line T24 and examined for potential stem cell characteristics related to proliferation, cell cycle distribution, self-renewal and differentiation. It was observed that T24 side population cells have stronger proliferative and colony formation abilities than non-side population cells. Side population cells were also more resistant to chemotherapy and radiotherapy, which may be due to expression of the ATP-binding cassette half-transporter, sub-family G, member 2 protein. Overall, the results suggest that side population cells from the human bladder transitional cell cancer cell line T24 harbour stem-like cells.
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