期刊
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
卷 56, 期 11, 页码 1064-1079出版社
WILEY
DOI: 10.1111/jipb.12167
关键词
Antioxidant; bermudagrass; calcium; chilling; freezing; metabolite; proteomic
资金
- Hundred Talents Program
- Knowledge Innovative Key Program of the Chinese Academy of Sciences [54Y154761O01076, Y329631O0263]
- National Natural Science Foundation of China [31200194]
- outstanding young talent program of Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture [Y352811O03]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
As an important second messenger, calcium is involved in plant cold stress response, including chilling (<20 degrees C) and freezing (<0 degrees C). In this study, exogenous application of calcium chloride (CaCl2) improved both chilling and freezing stress tolerances, while ethylene glycol-bis-(-aminoethyl) ether-N,N,N,N-tetraacetic acid (EGTA) reversed CaCl2 effects in bermudagrass (Cynodon dactylon (L.) Pers.). Physiological analyses showed that CaCl2 treatment alleviated the reactive oxygen species (ROS) burst and cell damage triggered by chilling stress, via activating antioxidant enzymes, non-enzymatic glutathione antioxidant pool, while EGTA treatment had the opposite effects. Additionally, comparative proteomic analysis identified 51 differentially expressed proteins that were enriched in redox, tricarboxylicacid cycle, glycolysis, photosynthesis, oxidative pentose phosphate pathway, and amino acid metabolisms. Consistently, 42 metabolites including amino acids, organic acids, sugars, and sugar alcohols were regulated by CaCl2 treatment under control and cold stress conditions, further confirming the common modulation of CaCl2 treatment in carbon metabolites and amino acid metabolism. Taken together, this study reported first evidence of the essential and protective roles of endogenous and exogenous calcium in bermudagrass response to cold stress, partially via activation of the antioxidants and modulation of several differentially expressed proteins and metabolic homeostasis in the process of cold acclimation.
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