期刊
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
卷 56, 期 3, 页码 315-332出版社
WILEY
DOI: 10.1111/jipb.12144
关键词
High-affinity potassium transporter (HKT); Na+; K+ ratio; Na+ stress; salt tolerance; Sorghum bicolor
资金
- One Hundred Talents Program of the Chinese Academy of Sciences [KSCX2-YW-G-067]
- National Science Foundation [Y4643A1001]
In halophytic plants, the high-affinity potassium transporter HKT gene family can selectively uptake K+ in the presence of toxic concentrations of Na+. This has so far not been well examined in glycophytic crops. Here, we report the characterization of SbHKT1;4, a member of the HKT gene family from Sorghum bicolor. Upon Na+ stress, SbHKT1;4 expression was more strongly upregulated in salt-tolerant sorghum accession, correlating with a better balanced Na+/K+ ratio and enhanced plant growth. Heterogeneous expression analyses in mutants of Saccharomyces cerevisiae and Arabidopsis thaliana indicated that overexpressing SbHKT1;4 resulted in hypersensitivity to Na+ stress, and such hypersensitivity could be alleviated with the supply of elevated levels of K+, implicating that SbHKT1;4 may mediate K+ uptake in the presence of excessive Na+. Further electrophysiological evidence demonstrated that SbHKT1;4 could transport Na+ and K+ when expressed in Xenopus laevis oocytes. The relevance of the finding that SbHKT1;4 functions to maintain optimal Na+/K+ balance under Na+ stress to the breeding of salt-tolerant glycophytic crops is discussed.
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