期刊
JOURNAL OF INSECT PHYSIOLOGY
卷 59, 期 3, 页码 273-282出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jinsphys.2012.11.005
关键词
Apis cerana cerana; AccTpx-3; Q-PCR; Oxidative stress; Antioxidant enzyme
资金
- earmarked fund for China Agriculture Research System [CARS-45]
- special fund for Agro-scientific Research in the Public Interest [200903006]
- National Natural Science Foundation in China [31172275]
Thioredoxin peroxidase (Tpxs) plays an important role in maintaining redox homeostasis and in protecting organisms from the accumulation of toxic reactive oxygen species (ROS). Here, we isolated a mitochondrial thioredoxin peroxidase gene from Apis cerana cerana, AccTpx-3. The open reading frame (ORE) of AccTpx-3 is 729 bp in length and encodes a predicted protein of 242 amino acids, 27.084 kDa and an isoelectric point of 8.70. Furthermore, the 980 bp 5' flanking region was cloned, and the transcription factor binding sites were predicted. A quantitative RT-PCR (Q-PCR) analysis indicated that AccTpx-3 was expressed higher in muscle than other tissues, with its highest expression occurring on the fourth day of the larval stage, followed by the fifteenth day of the adult stage. Moreover, the expression of the AccTpx-3 transcript was upregulated by such abiotic stresses as 4 degrees C, 42 degrees C, H2O2, cyhalothrin, acaricide and phoxime treatments. In contrast, AccTpx-3 transcription was downregulated by other abiotic stresses, including 16 degrees C, 25 degrees C, ultraviolet light and HgCl2. Recombinant AccTpx-3 protein acted as a potent antioxidant that resisted paraquat-induced oxidative stress and protected DNA from oxidative damage. Taken together, these results suggest that the AccTpx-3 protein is an antioxidant enzyme that may protect organisms from oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.
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