期刊
JOURNAL OF INSECT PHYSIOLOGY
卷 58, 期 8, 页码 1122-1127出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jinsphys.2012.05.011
关键词
Olfactory receptor; Olfaction; ME; RNAi; Bactrocera dorsalis
资金
- China Postdoctoral Science Foundation [5220112958]
- New Faculty in Huazhong Agricultural University [5220410191]
- International Foundation of Science [C/5149-1]
- Special Fund for Agro-scientific Research in the Public Interest [200903047]
- earmarked fund for Modern Agro-industry Technology Research System [CARS-27]
Bactrocera dorsalis is a destructive fruit-eating pest that causes severe economic damage to the fruit and vegetable industry. Methyl eugenol (ME) has been widely used as an effective sexual attractant for male fruit flies through olfactory perception. However, the molecular mechanism underlying the olfactory perception of ME remains unknown. Here, we report the characterization and functional analysis of a newly discovered cDNA that encodes a Drosophila melanogaster odorant receptor co-receptor Orco ortholog in B. dorsalis. qRT-PCR analysis revealed that it was abundantly expressed in the antenna of adult B. dorsalis. Notably, Orco was upregulated by ME in the antenna of male flies. Mature males of B. dorsalis showed significant taxis toward ME within 0.5 h, and Orco was significantly upregulated in the attracted adults within the same period. Silencing Orco through the ingestion of dsRNA reduced the attractive effects of ME. These data suggest that Orco may play an essential role in ME attraction in the olfactory signal transduction pathway. (C) 2012 Elsevier Ltd. All rights reserved.
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