期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 140, 期 -, 页码 64-71出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2014.07.003
关键词
Ruthenium complexes; G-quadruplex DNA; Light switch; Naked-eye and reversible; Molecular docking
资金
- National Natural Science Foundation of China [20901060, 31170776, 81171646]
- Fundamental Research Funds for the Central Universities
A pair of symmetrical furyl based ruthenium(II) complexes ([Ru(phen)(2)dpq-df](2+) (1) and [Ru(bpy)(2)dpq-df](2+) (2) (phen = 1,10-phenanthroline, bpy = 2,2'-bipyridine, dpq-df = dipyrido (3,2-a:2',3'-c) quinoxaline-difuran) have been prepared and characterized. The binding properties of both complexes toward G-quadruplex DNA have been investigated by fluorescence spectroscopy, UV-Vis spectroscopy, circular dichroism (CD), fluorescence resonance energy transfer (FRET) melting assays and molecular docking studies. The experimental results indicated that both Ru-complexes exhibited a remarkable light switch effect in the presence of hybrid G-quadruplex DNA Interestingly, the light switch can be repeated off and on through the successive addition of Cu2+ ions and EDTA, and all these behaviors can be observed even by the naked eyes. Moreover, FRET melting assay revealed that both complexes could be potential stabilizers for G-quadruplex architectures. The computational studies not only confirmed that the two complex molecules bound to one G-quadruplex DNA molecule, but also explained the light switch effect. (C) 2014 Elsevier Inc. All rights reserved.
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