期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 117, 期 -, 页码 35-47出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2012.08.008
关键词
Quinolones; Copper(II) complexes; Crystal structures; Interaction with calf-thymus DNA; Interaction with albumins
资金
- Slovenian Research Agency (ARRS) [P1-0175]
Three novel copper(II) complexes with the second-generation quinolone antibacterial agents norfloxacin (nfH) and ofloxacin (ofloH) have been synthesized resulting in the complexes [Cu(nfH)(phen)Cl]Cl center dot 5H(2)O (1.5H(2)O), [Cu(nfH)(2)]Cl-2 center dot 6H(2)O (2 center dot 6H(2)O) and [Cu-II(ofloH)(2)][((CuCl2)-Cl-I)(2)] (3), respectively. The crystal structures of the complexes have been determined by X-ray crystallography revealing that the quinolones act as bidentate ligands coordinated to Cu(II) atom through the pyridone oxygen and a carboxylato oxygen. UV study of the interaction of the quinolones and the complexes with calf-thymus DNA (CT DNA) has shown that they can bind to CT DNA with [Cu-II(ofloxacin)(2)][((CuCl2)-Cl-I)(2)] exhibiting the highest binding constant to CT DNA. The cyclic voltammograms of the complexes in the presence of CT DNA solution have shown that the interaction of the complexes with CT DNA is mainly through electrostatic binding. DNA solution viscosity measurements have shown that the interaction of the compounds with CT DNA by classical intercalation may be ruled out. Competitive studies with ethidium bromide (EB) indicate that the complexes can partially displace the DNA-bound EB suggesting low to moderate competition with EB. Norfloxacin, ofloxacin and their copper complexes exhibit good binding propensity to human or bovine serum albumin protein having relatively high binding constant values. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据