期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 104, 期 2, 页码 186-192出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2009.10.019
关键词
Myoglobin; Cisplatin; Transplatin; Mass spectrometry; Comparison; Binding site
资金
- National Science Foundation EPSCoR
Mass spectrometric studies of the interactions of cisplatin and transplatin with myoglobin (Mb) provide information concerning interaction kinetics, Mb adduct identity, and cisplatin and transplatin binding sites on Mb. Although the Mb-cisplatin interaction is faster than the Mb-transplatin interaction, mono-adducts and diadducts were formed in both the interactions over 30 h. In order to locate the binding sites of cisplatin and transplatin on Mb, digests of free Mb, Mb-cisplatin and Mb-transplatin adducts were subjected to analysis by Fourier transform mass spectrometry (FT-MS). This analysis revealed that two fragment ions, 1313.27(5+) and 1316.68(5+), were obtained only from the Mb-cisplatin and Mb-transplatin adduct digests. Tandem mass spectrometry (MS/MS and MS3) of the 1313.27(5+) and 1316.68(5+) ions indicate that these ions arise from [Pt(NH3)](2+) and [Pt(NH3)(2)](2+), respectively, bound to peptide His97-Gly153. The product-ion spectra of the MS/MS and MS3 analyses of the 1313.275+ ion indicate a common binding site of cisplatin and transplatin on His116-His119 residues. The interactions of cisplatin and transplatin with a dipeptide His-Ser and the three dimensional (3D) structure of native Mb suggest that cisplatin and transplatin coordinate to His116 and His119. (C) 2009 Elsevier Inc. All rights reserved.
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