期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 104, 期 4, 页码 431-441出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2009.12.008
关键词
1,3-tpbd Complexes; Magnetism; DNA interaction; DNA cleavage
资金
- National Natural Science Foundation of China [20771063]
Four copper(II) complexes [Cu-2(1,3-tpbd)Cl-4]center dot EtOH (1), {[Cu-2(1,3-tpbd)(mu-Cl)2](ClO4)(2)(H2O)(4.5) (NaClO4)}(infinity) (2), [Cu-2(1,3-tpbd)(1,10-phen)(2)(H2O)(2)](ClO4)(4) (3) and [Cu-2(1,3-tpbd)(2,2'-bpy)(2)(H2O)(2)](ClO4)(4) (4) (1,3-tpbd = N,N,N',N'-tetrakis(2-pyridylmethyl)benzene-1,3-diamine) have been synthesized and characterized by X-ray single crystal structure analysis. Variable-temperature magnetic susceptibility studies (2-300 K) indicate the existence of antiferromagnetic coupling between the copper(II) ions in complexes 2 and 3. The interactions of the four complexes with calf thymus DNA (CT-DNA) have been investigated by UV absorption, fluorescent spectroscopy, circular dichroism spectroscopy, viscosity and cyclic voltammetry, and the modes of CT-DNA binding to the complexes have been proposed. Furthermore, DNA cleavage activities by the four complexes were performed in the presence and absence of external agents, the results indicate that their cleavage activities have been promoted in the presence of external agents. Mechanism investigation shows that the four complexes could cleave DNA through both oxidative and hydrolytic processes. In the four copper(II) complexes, complex 2 showed highest cleavage activity with the pseudo-Michaelis-Menten kinetic paraments k(cat) = 5.16 h(-1) and K-m = 3.6 x 10(-5) M. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据